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stemspan sfem-ii supplemented with stemspan erythroid expansion supplement  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc stemspan sfem-ii supplemented with stemspan erythroid expansion supplement
    Stemspan Sfem Ii Supplemented With Stemspan Erythroid Expansion Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspan+sfem+ii+stemspan+erythroid+expansion+supplement/erythroid+differentiation+media+stemspan+sfem+ii+supplemented+with+1%C3%97+stemspan+erythroid+expansion+supplement/pm40154481-902-14-23
    Average 90 stars, based on 1 article reviews
    stemspan sfem-ii supplemented with stemspan erythroid expansion supplement - by Bioz Stars, 2026-09
    90/100 stars

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    Isolation:

    Article Title: Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis.
    Article Snippet: .. PBMCs were isolated from blood using Histopaque-1077 (SigmaAldrich), cultured in StemSpan SFEM II with StemSpan Erythroid Expansion Supplement (Stem Cell Technologies), and transduced with CytoTune 2.0 Sendai Virus Reprogramming Kit (Thermo Scientific), according to manufacturers’ protocols. .. Emerging single colonies were manually passaged from day 21 onwards onto MEF seeded plates in hiPSC medium with 10 μM ROCK inhibitor Y-27632 to enhance viability up until passage 5, and then routinely passaged using 1 U/mL dispase (Gibco) at a ratio of 1:3 every 4–5 days, and incubated at 37̊C with 5% CO2. hiPSC medium included Advanced DMEM/F12, 20% KnockOut Serum Replacement, 1% Non-Essential Amino Acids, 1% GlutaMAX, 1% Penicillin-Streptomycin, 55 μM 2-mercaptoethanol, and 4 ng/mL bFGF.

    Cell Culture:

    Article Title: Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis.
    Article Snippet: .. PBMCs were isolated from blood using Histopaque-1077 (SigmaAldrich), cultured in StemSpan SFEM II with StemSpan Erythroid Expansion Supplement (Stem Cell Technologies), and transduced with CytoTune 2.0 Sendai Virus Reprogramming Kit (Thermo Scientific), according to manufacturers’ protocols. .. Emerging single colonies were manually passaged from day 21 onwards onto MEF seeded plates in hiPSC medium with 10 μM ROCK inhibitor Y-27632 to enhance viability up until passage 5, and then routinely passaged using 1 U/mL dispase (Gibco) at a ratio of 1:3 every 4–5 days, and incubated at 37̊C with 5% CO2. hiPSC medium included Advanced DMEM/F12, 20% KnockOut Serum Replacement, 1% Non-Essential Amino Acids, 1% GlutaMAX, 1% Penicillin-Streptomycin, 55 μM 2-mercaptoethanol, and 4 ng/mL bFGF.

    Transduction:

    Article Title: Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis.
    Article Snippet: .. PBMCs were isolated from blood using Histopaque-1077 (SigmaAldrich), cultured in StemSpan SFEM II with StemSpan Erythroid Expansion Supplement (Stem Cell Technologies), and transduced with CytoTune 2.0 Sendai Virus Reprogramming Kit (Thermo Scientific), according to manufacturers’ protocols. .. Emerging single colonies were manually passaged from day 21 onwards onto MEF seeded plates in hiPSC medium with 10 μM ROCK inhibitor Y-27632 to enhance viability up until passage 5, and then routinely passaged using 1 U/mL dispase (Gibco) at a ratio of 1:3 every 4–5 days, and incubated at 37̊C with 5% CO2. hiPSC medium included Advanced DMEM/F12, 20% KnockOut Serum Replacement, 1% Non-Essential Amino Acids, 1% GlutaMAX, 1% Penicillin-Streptomycin, 55 μM 2-mercaptoethanol, and 4 ng/mL bFGF.

    Virus:

    Article Title: Generation and characterization of human induced pluripotent stem cell line METUi001-A from a 25-year-old male patient with relapsing-remitting multiple sclerosis.
    Article Snippet: .. PBMCs were isolated from blood using Histopaque-1077 (SigmaAldrich), cultured in StemSpan SFEM II with StemSpan Erythroid Expansion Supplement (Stem Cell Technologies), and transduced with CytoTune 2.0 Sendai Virus Reprogramming Kit (Thermo Scientific), according to manufacturers’ protocols. .. Emerging single colonies were manually passaged from day 21 onwards onto MEF seeded plates in hiPSC medium with 10 μM ROCK inhibitor Y-27632 to enhance viability up until passage 5, and then routinely passaged using 1 U/mL dispase (Gibco) at a ratio of 1:3 every 4–5 days, and incubated at 37̊C with 5% CO2. hiPSC medium included Advanced DMEM/F12, 20% KnockOut Serum Replacement, 1% Non-Essential Amino Acids, 1% GlutaMAX, 1% Penicillin-Streptomycin, 55 μM 2-mercaptoethanol, and 4 ng/mL bFGF.



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